rabbit anti col3  (Proteintech)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Inhibiting Lysyl Oxidases prevents pathologic cartilage calcification.

doi: 10.1016/j.biopha.2023.116075

Figure Lengend Snippet: Fig. 6. Proposed mechanism based on the observed results. Increased LOX(L) expression and activity promotes cartilage calcification (Anx, Enpp1, Pit1, Pit2, ALP). Both classical LOX(L) role (matrix cross-links) and non-classical roles are involved. The latter encompass: increased chondrocyte hypertrophy (Runx2, COL10), increased fibrosis (Col1, Col3), increased inflammation (IL-6) and increased oxidative stress (ROS). Finally, cartilage calcification can in turn induce increased LOX (L) activity.

Article Snippet: Collagen 1 (COL1) and Collagen 3 (COL3) expression were evaluated using an anti-COL1 rabbit polyclonal antibody (Boster, PA2140–2) and an anti-COL3 rabbit polyclonal antibody (Origene, AP06517PU-N) respectively.

Techniques: Expressing, Activity Assay

Journal: Europace

Article Title: Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model

doi: 10.1093/europace/eus052

Figure Lengend Snippet: Polymerase chain reaction primers used for amplification of inflammation and fibrosis-related genes

Article Snippet: After blocking with 5% non-fat milk, the membrane was incubated with anti-rabbit COL1 antibody (sc-8784; Santa Cruz Biotechnology, Inc., CA, USA), anti-rabbit COL3 antibody (LSL-LB-1387; Cosmo Bio Co., Ltd., Japan) or anti-mouse GAPDH antibody (GTX28245; GeneTex ® Inc., USA) and subsequently with the secondary antibody (Amersham ECL Plus Western blotting reagent pack; GE Healthcare UK Ltd.).

Techniques: Polymerase Chain Reaction, Amplification

Journal: Europace

Article Title: Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model

doi: 10.1093/europace/eus052

Figure Lengend Snippet: Messenger ribonucleic acid expressions of extracellular matrix and inflammation-related molecules. In the pacing control group, mRNA expressions of extracellular matrix-related genes (connective tissue growth factor, collagen type 1 and 3, and fibronectin 1) were up-regulated, especially in the right atrium in comparison with the non-pacing group, and this up-regulation was suppressed in the pacing + olmesartan group. In contrast, messenger ribonucleic acid expression of transforming growth factor-β did not differ among the three groups. See text for details. RA, right atrium; LA, left atrium; CTGF, connective tissue growth factor; COL1, collagen type 1; COL3, collagen type 3; FN1, fibronectin 1; TGF-β, transforming growth factor-β; MCP-1, monocyte chemotactic protein-1. * P < 0.05 vs. non-pacing group; † P < 0.05 vs. pacing control group.

Article Snippet: After blocking with 5% non-fat milk, the membrane was incubated with anti-rabbit COL1 antibody (sc-8784; Santa Cruz Biotechnology, Inc., CA, USA), anti-rabbit COL3 antibody (LSL-LB-1387; Cosmo Bio Co., Ltd., Japan) or anti-mouse GAPDH antibody (GTX28245; GeneTex ® Inc., USA) and subsequently with the secondary antibody (Amersham ECL Plus Western blotting reagent pack; GE Healthcare UK Ltd.).

Techniques: Control, Comparison, Expressing

Journal: Europace

Article Title: Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model

doi: 10.1093/europace/eus052

Figure Lengend Snippet: Western blot analysis of protein levels of collagens. ( A ) Representative examples of the bands and ( B ) a summary of all samples. Glyceraldehyde-3-phosphate dehydrogenase protein level was measured as an internal control. The protein level of collagen type 1 did not show any difference, but collagen type 3 was up-regulated in the pacing control group in comparison with the non-pacing group, and this up-regulation was suppressed in the pacing + olmesartan group. See text for details. RA, right atrium; LA, left atrium; COL1, collagen type 1; COL3, collagen type 3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. * P < 0.05 vs. non-pacing group; † P < 0.05 vs. pacing control group.

Article Snippet: After blocking with 5% non-fat milk, the membrane was incubated with anti-rabbit COL1 antibody (sc-8784; Santa Cruz Biotechnology, Inc., CA, USA), anti-rabbit COL3 antibody (LSL-LB-1387; Cosmo Bio Co., Ltd., Japan) or anti-mouse GAPDH antibody (GTX28245; GeneTex ® Inc., USA) and subsequently with the secondary antibody (Amersham ECL Plus Western blotting reagent pack; GE Healthcare UK Ltd.).

Techniques: Western Blot, Control, Comparison